Journal:

Article Title: Quality Control Guidelines for MIC Susceptibility Testing of Omiganan Pentahydrochloride (MBI 226), a Novel Antimicrobial Peptide

doi: 10.1128/JCM.42.3.1386-1387.2004

Figure Lengend Snippet: Proposed MIC QC ranges for omiganan listed by medium type a

Article Snippet: The proposed omiganan MIC QC ranges also were 1 log 2 dilution higher for CA-MHB when E. coli ATCC 25922 was used (Table ). table ft1 table-wrap mode="anchored" t5 caption a7 QC organism Omiganan MIC (μg/ml) QC range (% in range) for: CA-MHB MHB RPMI 1640 E. faecalis ATCC 29212 32-128 (100.0) 32-128 (100.0) S. aureus ATCC 29213 8-64 (99.5) 4-32 (100.0) S. pneumoniae ATCC 49619 b 32-128 (100.0) 16-64 (100.0) E. coli ATCC 25922 16-64 (99.0) 8-32 (100.0) P. aeruginosa ATCC 27853 64-256 (100.0) 8-64 (100.0) C. parapsilosis ATCC 22019 32-128 (99.0) C. krusei ATCC 6258 16-64 (100.0) Open in a separate window a RPMI 1640 was used for all tests of yeast. b Lysed horse blood (2 to 5%) was added to CA-MHB for testing this QC strain.

Techniques:

Journal: Acta Crystallographica. Section F, Structural Biology Communications

Article Title: The structure of a glycoside hydrolase 29 family member from a rumen bacterium reveals unique, dual carbohydrate-binding domains

doi: 10.1107/S2053230X16014072

Figure Lengend Snippet: Macromolecule-production information

Article Snippet: The protein was concentrated (at room temperature) using a 10 kDa molecular-weight cutoff ultrafiltration centrifugation column (Sartorius, Germany), further purified by gel filtration on a S200 16/60 size-exclusion column (GE Healthcare, Germany) in 50 m M Tris pH 7.5, 100 m M NaCl buffer and concentrated. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism Unknown DNA source Unknown Forward primer † Gene-specific att B1 primer as designed by GeneArt for Gateway cloning Reverse primer Gene-specific att B2 primer as designed by GeneArt for Gateway cloning Cloning vector pENTR221 Expression vector pDEST17 Expression host E. coli BL21 (DE3) Complete amino-acid sequence of the construct produced MSYYHHHHHHLESTSLYKKAGF ENLYFQS AQVEPCGPVPTENQLRWQDMEMYAFIHYSLNTYTDEEWGYGNEDPQLFNPSSLDCRQWARVCKQAGMRGIIFTAKHHCGFCMWPSAYTEYSVKNSPWKNGKGDVVRELADACREEGLKFAVYLSPWDRNHPAYGQPAYVAYFRNQLRELLTNYGEIFEVWFDGANGGDGWYGGANETRKIDRTTYYQWPETYKMIRQLQPNCLIWNDGSDRGDLRWVGTEAGNVGETNWSLLNHDGEVEWHMLHYGLENGDSWVPGETNTSIRPGWFYHDTENEHVKSLSKLMDTYYKSVGRNSTLLLNFPIAPNGRIHPNDSLRGIAFKKMIGEVFRKNLAEKARTQTKGDETVIDFGKPTTFNRFLAEEDIRYGQRVKKFLLEAEINGQWQQLKDALVENGDGLTTIGHRRIICFPTVNATKLRFTVVNTKCEPFIKKLGVYLAPELTADIPDAGEKKSSNLHLFFSSPTQMMIDWETEQTITSFRYLPPQESKDGTVTHYTLWASTDWSNWTKLASGEFSNVVNNPIWQTIKFQPVRAKILKLDADRLATGNRMAYGDVEVNLKLNIEN Open in a separate window † The att B1 forward primer contained an upstream rTEV cleavage site (underlined) caption a8 Macromolecule-production information

Techniques: Clone Assay, Plasmid Preparation, Expressing, Sequencing, Construct, Produced

Journal: Acta Crystallographica. Section F, Structural Biology Communications

Article Title: Neutron and high-resolution room-temperature X-ray data collection from crystallized lytic polysaccharide monooxygenase

doi: 10.1107/S2053230X15019743

Figure Lengend Snippet: Macromolecule-production information

Article Snippet: Details of the cloning and protein-production procedures are summarized in Table 1 . table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism J. denitrificans strain ATCC 14870 DNA source Synthetic DNA Forward primer 5-CCGTAGCAAT GGATCC ATGAAGAAGAGAAAGTTGAGAGCGTCAGC-3 Reverse primer 5-TCGTAATGCC GCGGCCGC TCATGAGACCACAACATCCATACAGTTG-3 Expression vector pUCBB-eGFP Expression host E. coli BL21 Star (DE3) Complete amino-acid sequence of the construct produced HGWVTDPPSRQALCASGETSFDCGQISYEPQSVEAPKGATTCSGGNEAFAILDDNSKPWPTTEIASTVDLTWKLTAPHNTSTWEYFVDGQLHQTFDQKGQQPPTSLTHTLTDLPTGEHTILARWNVSNTNNAFYNCMDVVVS Open in a separate window caption a8 Macromolecule-production information

Techniques: Expressing, Plasmid Preparation, Sequencing, Construct, Produced

Journal: Acta Crystallographica. Section F, Structural Biology Communications

Article Title: Crystal structure of the aromatic-amino-acid aminotransferase from Streptococcus mutans

doi: 10.1107/S2053230X18018472

Figure Lengend Snippet: Macromolecule-production information

Article Snippet: Macromolecule-production information is summarized in Table 1 . table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism S. mutans DNA source S. mutans strain UA159 (ATCC 700610) Forward primer 5′-CGC GGATCC ATGGATTTGAGTAAACGTTTTA-3′ Reverse primer 5′-CCG CTCGAG TTAGTCTGCATATTGCTCC-3′ Cloning vector pGEX-6P-1 Expression vector pGEX-6P-1 Expression host E. coli strain BL21 (DE3) Complete amino-acid sequence of the construct produced GPMDLSKRFNKNLNKIEVSMIRQFDQSISDIPDVLKLTLGEPDFATPKHIKEAAKRAIDADESHYTGMAGLLALRQAASAFVKEKYHLTYNPDNEILVTIGATEALSASLTAILEPGDKVLLPAPAYPGYEPVVNLVGAEVVEIDTRSNDFVLTPEMLEEAILKEGEALKAVILNYPTNPTGVTYSRQQIKNLAEVLKKYPIFVISDEVYAELTYTGESHVSIAEYLPDQTILISGLSKSHAMTGWRLGLIFAPAVLTAQLIKSHQYLVTAATTSVQFAAIEALTNGKDDALPMKEEYIKRRDYIIEKMEAMKFKIIKPDGAFYIFAKIPVAQGQDSFKFLQDFAKEKAVAFIPGVAFGKYGEGYLRISYAASMETIKEAMKRLKEFMEQYAD Open in a separate window caption a8 Macromolecule-production information 2.2.

Techniques: Cloning, Plasmid Preparation, Expressing, Sequencing, Construct, Produced

Journal: Molecular imaging and biology

Article Title: The Impact of Positron Range on PET Resolution, Evaluated with Phantoms and PHITS Monte Carlo Simulations for Conventional and Non-conventional Radionuclides

doi: 10.1007/s11307-019-01337-2

Figure Lengend Snippet: Simulation organ volumes, organ uptake values from Holland et al. used in simulations, and corresponding organ-level mean signal recovery in the modified Digimouse phantom, for different radionuclides

Article Snippet: Note in particular the poor quantitative accuracy provided by Ga-68 and As-72 for small structures, e.g ., tumor, spleen. table ft1 table-wrap mode="anchored" t5 Table 4. caption a7 Source tissue Source %ID/cc Volume (cc) Whole-organ mean signal recovery (%ID/cc) l RT ⊗ PSF scanner 52 Mn 18 F 89 Zr 86 y 68 Ga 72 As Tumor 42.4 0.06304 29.09 27.84 27.60 24.95 18.65 14.35 9.630 Brain 0.84 0.3651 3.111 3.118 3.124 3.169 3.116 3.103 2.767 Heart 1.79 0.1754 1.654 1.655 1.649 1.668 1.738 1.828 1.978 Kidney (L) 5.36 0.2085 4.698 4.631 4.616 4.454 4.005 3.680 3.197 Kidney (R) 5.36 0.2076 4.669 4.580 4.565 4.380 3.880 3.544 3.038 Liver 11.8 1.059 10.07 9.903 9.873 9.504 8.428 7.757 6.481 Lung (L) 0.66 0.1028 1.090 1.090 1.098 1.205 1.468 1.686 1.720 Lung (R) 0.66 0.1998 1.009 0.9931 0.9974 1.038 1.143 1.230 1.261 Pancreas 0.84 0.01359 2.747 2.948 2.993 3.389 3.661 3.912 3.417 Spleen 25.8 0.1082 15.17 14.38 14.23 12.64 9.497 7.498 5.436 Stomach 0.84 0.1832 1.536 1.640 1.661 1.904 2.477 2.891 3.067 Skeleton 13.7 0.643 7.578 7.404 7.372 6.984 6.005 5.369 4.414 Rem. body 0.84 18.46 1.382 1.382 1.383 1.398 1.407 1.416 1.189 Open in a separate window Simulation organ volumes, organ uptake values from Holland et al. used in simulations, and corresponding organ-level mean signal recovery in the modified Digimouse phantom, for different radionuclides

Techniques: Modification

Journal: Acta Crystallographica. Section F, Structural Biology Communications

Article Title: Neutron crystallographic study of heterotrimeric glutamine amidotransferase CAB

doi: 10.1107/S2053230X19000220

Figure Lengend Snippet: Macromolecule-production information (Nakamura et al. , 2006 ▸ , 2010 ▸ )

Article Snippet: Macromolecule-production information is given in Table 1 . table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism S. aureus Mu50 Cloning site NcoI/XhoI Expression vector pET-28b(+) vector (Novagen) Expression host E. coli B834 strain Complete amino-acid sequence of the construct produced GatA MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFLALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPIYESTVMEKLHKENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAAAVAAGLVPLSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTRNVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDVKEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSKEAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKVFENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRPIGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL GatB MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGENGYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSPKEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFNYVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHGADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLKQELDKRLEHHHHHH GatC MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDSADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA Open in a separate window caption a8 Macromolecule-production information (Nakamura et al. , 2006 , 2010 ) 2.2.

Techniques: Clone Assay, Expressing, Plasmid Preparation, Sequencing, Construct, Produced